ABSTRACT
Gene amplification requires a biological sample which is collected prior to the demands. The sample for such analysis plays a vital role as they serve as a resource for the core material-Deoxyribonucleic acid. A sample for gene amplification or any analysis would be collected prior to the demands and type of analysis. Human blood has been an essential resource of DNA from the commencement of DNA extraction in the 19th century. After then several protocols developed according to the requirement of both samples of different forms and their retrieval as various forms and methods. The sample has various characteristics and prerequisites when collected for gene analysis. Some important characteristics of sample collection methods are significant, which are not widely taken. This work analyses various general sample collection protocols and highlights some of the major characteristics and prerequisites for the sample. This work initiates and delivers to attain the core of genomics by bringing out the basic raw materials importance and consequences on amplification which is highly sensitive.
ABSTRACT
Live worm in anterior chamber is a rare finding. We hereby report a case of ocular Angiostrongylus cantonensis, which, to the best of our knowledge, is the third case report from India. A 70-year-old female presented with the complaints of watering and foreign body sensations in right eye since 2 months. On examination, a translucent worm of approximately 15 mm length was found freely moving in anterior chamber. Patient underwent surgical removal of live worm, which was identified as A. cantonensis. Educating people regarding importance of hygiene is important for prevention of worm infestation.
ABSTRACT
Worldwide, the overall prevalence of an ano rectal disease in the general population is estimated to be 4.4%. The faulty dietary pattern and life style , hereditary factors are important etiological factors of this disease. As per saint Yugi Moolam is classified as 21 types based on clinical symptoms and size, shape, colour of the pile mass which includes some of the ano-rectal diseases. The diet and deeds which influences the vital humors Vatham (Intestinal peristaltic movement ) and Pitham (metabolism and absorption) of food stuffs are said to be major causative factors of this disease. As per Yugi’s version the following ano rectal diseases under Moola noi have been categorized as, Neer moolam (Inflammatory bowel disease), Aazhi moolam (strangulated haemorrhoids), Sittru moolam (adenomatous colorectal polyps), Varal moolam (colorectal non- neoplastic polyps), Seezh moolam (kaposis sarcoma), Silaethuma moolam (anal warts condyloma), Thondha moolam (rectal cancer), Vinai moolam (irritable bowel syndrome), Powthira moolam (anal fistula), Kutha moolam (rectal prolapse), Ratha moolam (internal haemorrhoids 1st degree -bleeding piles), Mega moolam (gonococcal proctitis), Vaatha moolam (interno-external hemorrhoids), Pitha moolam (prolapsed haemorrhoids), Surukku moolam (anal stenosis), Mulai moolam (sentinel pile), Savvu moolam (perianal tuberclosis). This paper focuses the scientific basis of etiology and classification of Moolam by saint Yugi.